Perform complex experimental procedures, including:
- Growth of human endothelial cell cultures in single and pooled cultures.
- Exposing cultured cells to shear stress to mimic hemodynamic blood flow.
- Performing molecular biology techniques such as nucleic acid extractions, quantitative real-time PCR, RNA-seq, ATAC-seq, ChIP-seq, and other -seq assays including library preparation.
- Performing immunolabeling, immunoblotting, and confocal/high-content microscopy for cellular imaging.
- Transfect/infect cells with oligos and lentivirus in vitro for gain- and loss-of-function experiments.
Protocol Design and Refinements:
- With minimal supervisor guidance, refines, adapts, and makes decisions to switch experiment parameters in response to situational demands of experiments.
- Assists in preparing experimental designs, technical protocols and techniques, and Standard Operating Procedures (SOPs).
Record-keeping, Analysis, and Presentations:
- Communicates effectively all aspects of experiments with PI or other lab members and collaborators.
- Keeps accurate and detailed records of experiments, results, and enters data in laboratory databases and filesystems.
- Communicates with collaborating investigators and laboratories.
- Catalogs specimens and samples.
- Performs independent analysis and interpretation of data for investigator and team.
Operational Support:
- Orders and catalogs laboratory supplies and supports laboratory operations.
- Assist in maintaining laboratory equipment and related records.
- Operates and calibrates specialized scientific equipment including instruments for exposing cell cultures to fluid shear stress.
Knowledge, Skills & Abilities:
- Ability to maintain protocols, data, and samples organized with clear documentation recording how experiments were performed to ensure rigor and reproducibility.
- Ability to communicate clearly with all members of the team.
