- Plan, design and conduct advanced, multi-disciplinary research in collaboration with PI.
- Perform in vivo physiological examination to animals including, but not limited to: echocardiography, treadmill, Doppler, blood pressure. Isolation of mouse vessels, including the aorta, coronary artery, and perform vasodilation studies with myography.
- Manage the mouse colonies.
- Perform animal experiments (survival surgical procedure, e.g., open chest surgeries, contrast echocardiography, echocardiography, Doppler, treadmill, blood pressure measurement with a catheter,PV -loop) and data analysis. Measure the myocardial blood flow in vivo.
- Remain updated on literature of the related research topic and develop the projects aligning with the PI's research focus.
- Plan, coordinate, and conduct scientific research requiring judgment in the independent evaluation, selection, and substantial adaption and modification of standard scientific techniques.
- Design, perform, and/or oversee experiments and data collection to ensure data integrity, quality control, and protocol compliance.
- Report the experimental results along with original experiment data, protocol, analysis files (prism and ppt).
- Perform aspects related to cellular and molecular biology research that include, but are not limited to: bulk RNA-sequencing, scRNA-sequencing, bioinformatics, qRT-PCR, Immunoblotting, proteomics and metabolomics, and Immunocytofluorescence, primary cell isolation and culture, seahorse analysis, and flowcytometry.
- Attend and present research finding at regular group meetings and scientific conferences.
- Manage daily laboratory operations, including manage, maintain, and order laboratory equipment and supplies; lab inventories and respond to administrative request and procedures.
- Guide the work of others (students and trainees) and/or provides guidance to a specialized laboratory or research area.
- Serve as the bio-safety and chemical safety officer for TCRC, including the management of all lab safety and approval protocols.
- Develop and maintain detailed records of experiments, back the original data.
- Analyze data with statistical tools and writing findings narrative reports as appropriate.
- Prepare manuscripts for publication.
- Participate in the acquisition of research funding by initiating and writing grant proposals as appropriate.
- Assist with preparation of grants from the lab.
Knowledge, Skills, and Abilities:
- Knowledge of In vivo physiological animal studies.
- Knowledge of microvessel isolation and myograph.
- Knowledge of molecular and cellular biology.
- Skilled in grant writing and manuscript preparation.
