Scientist-II

The candidate will contribute to the production and functional assessment of chimeric antigen receptor T cells for the treatment of hematological malignancies. She/he will work to optimize multiple nucleic-acid delivery platforms for both primary and established T cell lines for the purpose of generating stable transfectants. The scientist will subsequently characterize the short- and long-term functionality of the product(s) via an array of cell-based platforms.

ESSENTIAL FUNCTIONS:

• The candidate will work to engineer multiple stably-transfected T cell lines using both mRNA- and pDNA-based delivery platforms. This effort will encompass extensive cell culture methodologies, as well as the ability to perform downstream analyses necessary to validate delivery of the transgene(s).

• A diverse background in mammalian cell culture is absolutely essential.

• Experience with primary T cells is preferred. The successful applicant should demonstrate extensive knowledge surrounding cell culture techniques, gene delivery platforms (i.e., transfection/transduction), and downstream functional validation of resultant cells.

• Experience with mammalian cell-based electroporation platforms (i.e., Amaxa, MaxCyte, BTX, etc.) is required. Preference will be given to candidates with expertise in flow cytometry, gene editing (i.e., CRISPR, transposase, etc.), multiplex ELISA, and viral-delivery techniques (i.e., AAV, lentivirus).

• The successful candidate will work independently, but cooperatively, within the larger group. The ability to generate and maintain experimental data in an electronic laboratory notebook in a highly organized manner is essential.

Education:

• M.S. in Biomedical Engineering, Molecular Biology, Cell biology, Biochemistry, Chemistry or a related field with 2+ years industry experience.

For more information, Please contact

Sneha Shrivastava

973-967-3348

sneha.shrivastava(at)artechinfo.com