The Research Assistant II assists the PI with scientific studies to elucidate the cellular mechanisms of neural network dysfunction in genetic models ofneurodevelopmental disorders. The Research Assistant is able to read and understand scientific literature, carry out experiments, work collaboratively, plan experiments, analyze data, draft the manuscript and make presentations to others about the work.
*Ideal candidate would have tissue culture experience, R, or Python, and some exposure to EEG
1. Perform tissue culture experiments using iPSC derived neural cells.2. Use methods such as fluorescent in situ hybridization, immunohistochemistry, Ca2+ imaging, and MEA recording.3. Conduct experiments in rodents, using surgical techniques, post-operative care, behavioral studies and euthanasia, in accordance with IACUC-approvedprotocols and the Guide for the Care and Use of Experimental Animals.4. Manage the colony of transgenic mice, obtaining samples for genotyping, maintaining accurate records of genotypes and breeding, and managingsupply of breeders and mice required for experiments.5. Use computerized microscopy and image analysis systems to collect experimental data; analyze data with statistics packages such as R andGraphPad Prism.6. Design and plan experiments, in coordination with the PI, using above/other methods.7. Work cooperatively with others in the laboratory to carry out collaborative research.
QUALIFICATIONS:• Experience in MATLAB, R and Python is preferred.• Training in animal surgical techniques, EEG placement and post-operative care is preferredEDUCATION:• Bachelor’s degree required, preferably in a science related field such as biology, neuroscience, psychology, biomedical engineering, computer science, or physics.EXPERIENCE:• Previous laboratory training and experience in any field of science is required.• Two years’ experience with tissue culture, molecular biology, is preferred.
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Kennedy Krieger Institute is a non-profit organization dedicated to improving the lives of children and adults with disorders of the brain, spinal cord, and musculoskeletal system.