Associate Scientist

Title: Associate Scientist
Duration: 12 months
Location: Cambridge, MA
Job Description
The Gene Therapy Accelerator Unit (GTxAU) at *** is seeking a committed, enthusiastic, and dependable scientist to contribute to the generation of gene therapy laboratory and clinical candidates for neurodegenerative and neuroinflammatory diseases.
The Associate Scientist II/III, cargo engineering, will work collaboratively with a cross-function team and growth potentials. The incumbent will be part of a team focused on cargo/AAV vector designing and characterization for in vitro and in vivo testing. An ideal candidate will have experience in mammalian cell culture, cell line engineering, molecular biology techniques, and biochemical/immunological assays. This candidate should be able to design, execute, and analyze in vitro experiments independently with strong scientific rigor. The ideal candidate should also maintain an up-to-date knowledge of pertinent literature and possess excellent communication and interpersonal skills to efficiently present results to project team members.
• AAV vector designing by adapting existing protocols, including titering assay by ddPCR, genome integrity assay, empty/full particle ratio assay, endotoxin assay, residual DNA and protein assays
• Maintain constant awareness of Client biochemical and biophysical technologies for AAV purification and characterization
• Work as a liaison between GTxAU and the upstream and downstream process teams
• Establish SOPs and adapt new protocols from literature
• Manage external CROs for plasmid or vector production
• Maintain electronic laboratory notebooks, present data to the team, and write study reports

Qualifications
• 2+ years of experience in biotech/pharmaceutical industry or academic setting
• Experience with mammalian cell culture and cell line engineering
• Hands-on experience with molecular biology techniques (cloning, qPCR, ddPCR, DNA gel electrophoresis, SDS-PAGE, WB, ELISA, etc.)
• Ability to independently design, execute, and analyze in vitro molecular biology experiments
• Critical thinking and presentation skills a must
• Strong communication and organizational skills
• Ability to productively collaborate in cross-function teams
Preferred:
• Hands-on experience with AAV and/or other gene therapy delivery systems
• Hands-on experience with immunological assays
• Previous research experience in neuroscience/neurological disorders

Education:
• BS or MS in scientific field (biology, biochemistry, biotechnology, etc.)

All your information will be kept confidential according to EEO guidelines.