- Shift Length: Variable
- Additional Shift Details: M-F variable hours
- Budgeted Job Salary Range: 28.96-30.23
- Campus Location 1: Parnassus Heights (SF)
- Bargaining Unit: RX
- Employee Class: Limited Appt
- FLSA Status: Non-exempt
- Job Code and Payroll Title: 009613
- Workplace: Fully On-Site
- FTE%: 40%
As an SRA I, you will play a vital role in Dr. Chou's research group, which focuses on evaluating the mechanisms of sensitivity and resistance to antibody drug conjugates (ADCs) and CAR T-cell therapies. The group has developed innovative experimental models and utilizes advanced sequencing techniques. Your primary responsibilities will involve validating research findings, conducting functional tests, and providing bioinformatics support.
Responsibilities
- 40%: Assists with bioinformatics analyses of transcriptomic and whole-exome sequencing data.
- 35%: Assists with sgRNA cloning to validate prior genetic screens, including ordering oligonucleotides, transforming bacteria with sgRNA constructs, and confirming sequences through Sanger sequencing.
- 15%: Performs validation of transcriptomic data using quantitative PCR (qPCR).
- 10%: Performs other related laboratory duties and responsibilities as assigned.
Qualifications
Required Qualifications:
- BA/BS in Biology or related as well as in Computational Biology/Computer Science with biology applications
- Experience in R Studio and/or Python to analyze single-cell RNA sequencing, bulk RNA sequencing, and whole-exome sequencing data
- Experience in molecular biology techniques such as sgRNA cloning and sequencing, gel purification, miniprep, and expression of lentiviruses using HEK293T/LentiX cells
- Experience in lentiviral generation, transduction, and validation techniques such as qPCR and/or flow cytometry
- Experience with Incucyte to monitor cell growth
- Awareness of different types of clinical research studies
- Willingness and ability to learn new methods and skills
- Ability to work independently and as a member of a research team
- Ability to demonstrate interpersonal communication skills
- Ability to demonstrate organizational skills; ability to prioritize tasks, coordinate work tasks with others, and meet multiple deadlines
- Ability to perform simple mathematical calculations
Preferred Qualifications:
- Prior experience with working in a wet lab and with computational biology experience
